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hek293 cells  (Revvity)


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    Revvity hek293 cells
    Hek293 Cells, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293 cells/product/Revvity
    Average 91 stars, based on 10 article reviews
    hek293 cells - by Bioz Stars, 2026-02
    91/100 stars

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    Representative HCA images showing drug-induced nephrotoxicity in Hoechst 33342, MitoTracker Deep Red FM, and rhodamine 123 channels. HEK293 cells treated with blank (a, e, i, m), 10 μ M aristolochic acid (AA) (b, f, j, n), 10 μ M cyclosporine A (CsA) (c, g, k, o), and 10 μ M cisplatin (CDDP) (d, h, l, p) are shown. Decreased intensity of rhodamine 123 indicates decreased mitochondrial membrane potential (MMP) (f, g, h). Increased intensity of MitoTracker Deep Red FM indicates increased mitochondrial mass (MS) (j, k, l). The number of cells stained by Hoechst 33342 was decreased by all three drugs (n, o, p).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity

    doi: 10.1155/2018/2461915

    Figure Lengend Snippet: Representative HCA images showing drug-induced nephrotoxicity in Hoechst 33342, MitoTracker Deep Red FM, and rhodamine 123 channels. HEK293 cells treated with blank (a, e, i, m), 10 μ M aristolochic acid (AA) (b, f, j, n), 10 μ M cyclosporine A (CsA) (c, g, k, o), and 10 μ M cisplatin (CDDP) (d, h, l, p) are shown. Decreased intensity of rhodamine 123 indicates decreased mitochondrial membrane potential (MMP) (f, g, h). Increased intensity of MitoTracker Deep Red FM indicates increased mitochondrial mass (MS) (j, k, l). The number of cells stained by Hoechst 33342 was decreased by all three drugs (n, o, p).

    Article Snippet: Operetta high content analysis (HCA, PerkinElmer, Waltham, MA, USA), which is an in vitro, sensitive, reproducible, multiparametric screening method, was used to evaluate the cytotoxicity of HMPs in cultures of HEK293 human embryo kidney cells.

    Techniques: Membrane, Staining

    Aristolochic acid (AA), cyclosporine A (CsA), and cisplatin (CDDP) dose-response curves for cell viability (a), mitochondrial mass (b), mitochondrial membrane potential (c), nuclear area (d), and nuclear roundness (e). HEK293 cells were exposed to 0.01, 0.1, 1, 10, and 100 μ M of the three drugs. Data are means ± SEM ( n = 4).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity

    doi: 10.1155/2018/2461915

    Figure Lengend Snippet: Aristolochic acid (AA), cyclosporine A (CsA), and cisplatin (CDDP) dose-response curves for cell viability (a), mitochondrial mass (b), mitochondrial membrane potential (c), nuclear area (d), and nuclear roundness (e). HEK293 cells were exposed to 0.01, 0.1, 1, 10, and 100 μ M of the three drugs. Data are means ± SEM ( n = 4).

    Article Snippet: Operetta high content analysis (HCA, PerkinElmer, Waltham, MA, USA), which is an in vitro, sensitive, reproducible, multiparametric screening method, was used to evaluate the cytotoxicity of HMPs in cultures of HEK293 human embryo kidney cells.

    Techniques: Membrane

    Representative images of the HCA analysis of HEK293 cells for the evaluation of cell number and morphology and stained by three fluorescence probes. Control (a, d, g, j), cyclosporine (CA) 0.01 μ M (b, e, h, k), and triptolide (TR) 0.01 μ M (c, f, i, l).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity

    doi: 10.1155/2018/2461915

    Figure Lengend Snippet: Representative images of the HCA analysis of HEK293 cells for the evaluation of cell number and morphology and stained by three fluorescence probes. Control (a, d, g, j), cyclosporine (CA) 0.01 μ M (b, e, h, k), and triptolide (TR) 0.01 μ M (c, f, i, l).

    Article Snippet: Operetta high content analysis (HCA, PerkinElmer, Waltham, MA, USA), which is an in vitro, sensitive, reproducible, multiparametric screening method, was used to evaluate the cytotoxicity of HMPs in cultures of HEK293 human embryo kidney cells.

    Techniques: Staining, Fluorescence, Control

    Change of cell viability (CV), nucleus area (NA), nucleus roundness (NR), mitochondrial mass (MS), and mitochondrial membrane potential (MMP) produced in HEK293 cells by 18 compounds after 24 and 48 h culture. Data are means ± SEM ( n = 3). ∗ P < 0.05. ∗∗ P < 0.01 compared with controls (BLANK).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity

    doi: 10.1155/2018/2461915

    Figure Lengend Snippet: Change of cell viability (CV), nucleus area (NA), nucleus roundness (NR), mitochondrial mass (MS), and mitochondrial membrane potential (MMP) produced in HEK293 cells by 18 compounds after 24 and 48 h culture. Data are means ± SEM ( n = 3). ∗ P < 0.05. ∗∗ P < 0.01 compared with controls (BLANK).

    Article Snippet: Operetta high content analysis (HCA, PerkinElmer, Waltham, MA, USA), which is an in vitro, sensitive, reproducible, multiparametric screening method, was used to evaluate the cytotoxicity of HMPs in cultures of HEK293 human embryo kidney cells.

    Techniques: Membrane, Produced